产生IgM单克隆抗体的小鼠-小鼠杂交瘤的培养。
Cultivation of mouse-mouse hybridomas producing IgM monoclonal antibodies.
作者信息
Knezević M, Alscher M, Zunec P, Hartman-Pretnar K, Rozman P, Curin-Serbec V
机构信息
BIA Ltd., Ljubljana, Slovenia.
出版信息
Pflugers Arch. 1996;431(6 Suppl 2):R231-2.
The mouse-mouse hybridomas producing monoclonal antibodies (MAbs) for ABO blood group determination were cultivated for 10 days in 25 cm2 Roux bottles using standard cultivation medium (DMEM) with different foetal-calf serum (FCS) concentrations (2-13%). The highest specific production rates (200-1100 micrograms/10(6) cells/day) for MAbs were measured at the end of the cultivation: this phenomenon could be explained by advanced cell death and liberating the content of the cells into the medium.
用于ABO血型测定的产生单克隆抗体（MAb）的小鼠-小鼠杂交瘤，在25 cm²鲁氏瓶中使用含有不同胎牛血清（FCS）浓度（2-13%）的标准培养基（DMEM）培养10天。在培养结束时测量到单克隆抗体的最高比生产率（200-1100微克/10⁶细胞/天）：这种现象可以通过细胞晚期死亡并将细胞内容物释放到培养基中来解释。
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