Strus J, Compere P
Department of Biology, University of Ljubljana, Slovenia.
Pflugers Arch. 1996;431(6 Suppl 2):R251-2. doi: 10.1007/BF02346363.
The formation of the cuticle was investigated during moulting of the isopod crustacean Ligia italica. The intermoult cuticle is a four-layered lamellar structure composed of chitin-protein fibrils and mineralized in its upper half. The distribution of calcium carbonate in cuticle during moult cycle was determined by cytochemical methods and X-ray microanalysis, Epi-and exocuticle are secreted during premoult. Calcium is resorbed from the old cuticle and accumulates in the ecdysal gap as calcium granules. Endocuticle is secreted after moult when the mineralization of exocuticle starts. The shape and ultrastructure of epithelial cells change during cuticle secretion and mineralization. Mitochondria, bundles of filaments, calcium granules and large amounts of glycogen accumulate in the apical cytoplasm of cells in premoult animals.
在意大利潮虫等足类甲壳动物蜕皮过程中，对其角质层的形成进行了研究。蜕皮间期的角质层是一种四层的片状结构，由几丁质 - 蛋白质原纤维组成，并且在上半部分矿化。通过细胞化学方法和X射线微分析确定了蜕皮周期中角质层内碳酸钙的分布。上表皮和外表皮在蜕皮前分泌。钙从旧角质层中被重新吸收，并以钙颗粒的形式积聚在蜕皮间隙中。蜕皮后，当外表皮开始矿化时，内表皮开始分泌。在角质层分泌和矿化过程中，上皮细胞的形状和超微结构会发生变化。在蜕皮前的动物细胞顶端细胞质中，线粒体、丝状束、钙颗粒和大量糖原会积累。
