Serpe M D, Nothnagel E A
Department of Botany and Plant Sciences-072, University of California, Riverside 92521-0124, USA.
Pflugers Arch. 1996;431(6 Suppl 2):R253-4. doi: 10.1007/BF02346364.
Fluorescence photobleaching recovery was used to measure the apparent lateral diffusion coefficient and mobile fraction of a fluorescent lipid probe in the plasma membranes of whole plant cells, i.e., in the presence of cell walls. Interfering fluorescence from the cell wall was reduced by extensive washing and then subtracted from the recovery recordings. Mobility characteristics of plasma membrane lipids in whole cells were found to be very similar to those in protoplasts.
荧光光漂白恢复技术被用于测量荧光脂质探针在完整植物细胞质膜中的表观侧向扩散系数和移动分数，即在有细胞壁存在的情况下。通过大量洗涤减少来自细胞壁的干扰荧光，然后从恢复记录中减去。发现完整细胞中质膜脂质的流动性特征与原生质体中的非常相似。
