Ruiz J E, Kwak J Y, Baum L, Gilman-Sachs A, Beaman K D, Kim Y B, Beer A E
Department of Microbiology and Immunology, Finch University of Health Sciences, Chicago Medical School, IL 60064, USA.
Am J Reprod Immunol. 1996 Apr;35(4):370-5. doi: 10.1111/j.1600-0897.1996.tb00496.x.
We previously reported elevation of natural killer (NK) cells in women with recurrent spontaneous abortion (RSA) of immune etiology. In this study, we investigated the effect of intravenous immunoglobulin G (IVIg) on peripheral blood NK activity in vivo in women with RSA. Blood was drawn prior to and 7-11 days after IVIg therapy in eight women with RSA. NK activity was measured using K562 as target cells for 51Cr-release assays. Serum IgG concentrations were also measured. All received 400 mg/kg/day of IVIg for 3 consecutive days. 1) Seven of eight women became pregnant. Five delivered a live born infant. Three out of five women (60%) who delivered a live born infant showed a significant inhibition of NK cytotoxicity post IVIg and the rest did not show any changes; 2) NK cytotoxicity was significantly increased in a woman who miscarried again; 3) A woman who miscarried a chromosomally abnormal fetus showed a significant inhibition of NK cytotoxicity after IVIg; and 4) Serum IgG concentration increased significantly from 9.3 +/- 3.0 mg/ml to 23.5 +/- 5.1 mg/ml post IVIg therapy. IVIg effectively inhibits peripheral blood NK activity in vivo. These results are consistent with our previous finding showing that IVIg inhibits NK cell activity in vitro. Women with RSA and elevated NK cells may benefit from IVIg treatment.
我们之前报道过，免疫病因导致复发性自然流产（RSA）的女性体内自然杀伤（NK）细胞水平升高。在本研究中，我们调查了静脉注射免疫球蛋白G（IVIg）对RSA女性体内外周血NK活性的影响。在8名RSA女性接受IVIg治疗前及治疗后7 - 11天采集血液。使用K562作为靶细胞通过51Cr释放试验测量NK活性。同时也测量血清IgG浓度。所有患者均连续3天接受400 mg/kg/天的IVIg治疗。1）8名女性中有7名怀孕。5名分娩出活产婴儿。5名分娩出活产婴儿的女性中有3名（60%）在IVIg治疗后NK细胞毒性受到显著抑制，其余未显示任何变化；2）再次流产的1名女性NK细胞毒性显著增加；3）流产出染色体异常胎儿的1名女性在IVIg治疗后NK细胞毒性受到显著抑制；4）IVIg治疗后血清IgG浓度从9.3±3.0 mg/ml显著增加至23.5±5.1 mg/ml。IVIg可有效抑制体内外周血NK活性。这些结果与我们之前在体外实验中发现IVIg抑制NK细胞活性的结果一致。RSA且NK细胞水平升高的女性可能从IVIg治疗中获益。
