Sarris A H, Palade G E
J Biol Chem. 1979 Jul 25;254(14):6724-31.
Murine erythrocyte ghosts (from DBA/2, CD-1, and B6D2 strains) contain significant amounts of O-acetylated sialyl residues, which are slowly oxidized by periodate. Sialic acids have been purified from murine erythrocyte ghosts and the existence of O-acetylated sialic acids has been confirmed: 1) by assaying with the Warren procedure before and after de-O-acetylation with 0.1 N NaOH for 45 min at 4 degrees C; 2) by thin layer chromatography on cellulose; and 3) by gas-liquid chromatography. Because these sialyl residues are unevenly distributed on the sialoglycoproteins of murine erythrocyte ghosts, the periodic acid-Schiff staining detects only one major sialoglycoprotein. A modification of the periodic acid-Schiff stain method removes these O-acetyl groups after electrophoresis and reveals two additional sialoglycoproteins which bear the majority of the O-acetylated sialyl residues. Rat erythrocyte ghosts have similar residues on one of their two sialoglycoproteins. Ghosts of human, rabbit, and guinea pig erythrocytes do not contain detectable amounts of O-acetylated sialyl residues.
小鼠红细胞血影（来自DBA/2、CD-1和B6D2品系）含有大量的O-乙酰化唾液酸残基，这些残基可被高碘酸盐缓慢氧化。已从小鼠红细胞血影中纯化出唾液酸，并证实了O-乙酰化唾液酸的存在：1）通过在4℃下用0.1N NaOH进行45分钟的脱O-乙酰化前后，采用沃伦法进行测定；2）通过在纤维素上进行薄层层析；3）通过气-液色谱法。由于这些唾液酸残基在小鼠红细胞血影的唾液糖蛋白上分布不均，高碘酸-希夫染色仅检测到一种主要的唾液糖蛋白。高碘酸-希夫染色方法的一种改进是在电泳后去除这些O-乙酰基，并揭示出另外两种带有大部分O-乙酰化唾液酸残基的唾液糖蛋白。大鼠红细胞血影在其两种唾液糖蛋白中的一种上有类似的残基。人、兔和豚鼠红细胞的血影不含可检测量的O-乙酰化唾液酸残基。
