Piepkorn M
Department of Medicine (Dermatology), University of Washington School of Medicine, Seattle 98195, USA.
Am J Dermatopathol. 1996 Apr;18(2):165-71. doi: 10.1097/00000372-199604000-00010.
Previous studies have indicated that amphiregulin is a major autocrine growth factor for cultured human keratinocytes. Its overexpression could therefore be important in hyperproliferative skin diseases. The purpose of this preliminary study was to determine if there is upregulation of amphiregulin protein in those disorders. A variety of lesions was surveyed for qualitative alterations in its immunostaining with an anti-amphiregulin monoclonal antibody. Amphiregulin was barely detectable in the epidermis of normal controls, although there was random nuclear staining of keratinocytes, and the epidermal appendages, especially sebaceous glands, were usually reactive. In contrast, psoriatic lesions exhibited prominent cytoplasmic staining of basal and spinous keratinocytes. Somewhat increased reactivity was also evident in actinic keratoses, in nests of squamous carcinoma cells, and in verrucae. Adnexal tumors were often strongly stained. Whereas basal cell carcinomas were nonreactive, staining was present in adjacent epidermis. Similarly, the melanocytes of nevi and melanoma were nonreactive but there was increased staining in contiguous keratinocytes. The pattern of amphiregulin immunostaining suggests a role for the protein in the aberrant keratinocyte growth of hyperproliferative disorders.
以往的研究表明，双调蛋白是培养的人角质形成细胞的主要自分泌生长因子。因此，其过表达在增殖性皮肤病中可能具有重要意义。本初步研究的目的是确定在这些疾病中双调蛋白是否上调。用抗双调蛋白单克隆抗体检测了多种病变中其免疫染色的定性改变。在正常对照的表皮中几乎检测不到双调蛋白，尽管角质形成细胞有随机的核染色，且表皮附属器，尤其是皮脂腺，通常有反应。相比之下，银屑病皮损中基底和棘层角质形成细胞呈现明显的胞质染色。光化性角化病、鳞状癌细胞巢和疣中也有明显增强的反应性。附属器肿瘤常被强烈染色。基底细胞癌无反应，但相邻表皮有染色。同样，痣和黑色素瘤的黑素细胞无反应，但相邻角质形成细胞的染色增加。双调蛋白免疫染色模式表明该蛋白在增殖性疾病中异常角质形成细胞生长中起作用。
