Goldring S R, Dayer J M, Russell R G, Mankin H J, Krane S M
J Clin Endocrinol Metab. 1978 Mar;46(3):425-33. doi: 10.1210/jcem-46-3-425.
Giant cell tumors of bone obtained from 7 patients were dispersed with clostridial collagenase and trypsin and adherent cells were maintained in culture. Early cultures contained both mononucleated and multinucleated cells presumably derived from the stromal and giant cells of the original tumor. The original multinucleated cells did not survive for greater than 7-10 days whereas the mononucleated cells persisted and could be passaged by trypsinization. In 5 of 7 early cultures exposed to parathyroid hormone (PTH) there was a rise in cAMP within 5-10 min in both cells and medium which averaged approximately 12-fold. None of the cells responded to calcitonin and a variable rise in cAMP was seen after incubation with prostaglandin E2. In cells cultured from 3 tumors the PTH response disappeared with passage of the cells, but in the remaining 2, PTH response persisted through multiple passages. The presence as well as the magnitude of the PTH-induced cAMP response in these cells is consistent with a skeletal origin.
从7例患者获取的骨巨细胞瘤用梭菌胶原酶和胰蛋白酶进行分散处理，贴壁细胞进行培养。早期培养物中既有单核细胞也有多核细胞，推测它们分别来源于原始肿瘤的基质细胞和巨细胞。原始的多核细胞存活时间不超过7 - 10天，而单核细胞持续存在，并可通过胰蛋白酶消化进行传代。在7个早期培养物中有5个暴露于甲状旁腺激素（PTH），细胞和培养基中的环磷酸腺苷（cAMP）在5 - 10分钟内均升高，平均约为12倍。所有细胞对降钙素均无反应，与前列腺素E2孵育后cAMP出现不同程度的升高。在来自3个肿瘤的培养细胞中，随着细胞传代，PTH反应消失，但在其余2个中，PTH反应在多次传代后仍持续存在。这些细胞中PTH诱导的cAMP反应的存在及其程度与骨骼来源一致。
