Kerboeuf D, Aycardi J, Soubieux D
INRA, Station de Pathologie Aviaire et de Parasitologie, Nouzilly, France.
Parasitol Res. 1996;82(4):358-63. doi: 10.1007/s004360050126.
Flow cytometry was applied to the analysis of nematode populations. Three strains of Haemonchus contortus susceptible or resistant to anthelmintics were studied. Eggs were chosen for these analyses. Data on light-scatter emissions and native green fluorescence were collected. In addition, the size of the eggs (image analysis), the hatching rate, and the susceptibility to benzimidazoles were measured. The results showed that nematode eggs are a suitable material for multiparametric flow-cytometry analyses. Forward-scatter emission is a discriminating parameter for the egg size. The hatching rate and side-scatter emission have a significantly positive relationship. For resistant strains, the rate of resistance shows a significant regression on the native greenfluorescence pulses that might reflect the state of oxidation of associated flavin molecules.
流式细胞术被应用于线虫种群分析。研究了三种对驱虫药敏感或耐药的捻转血矛线虫菌株。选取虫卵进行这些分析。收集了关于光散射发射和天然绿色荧光的数据。此外，还测量了虫卵大小（图像分析）、孵化率以及对苯并咪唑的敏感性。结果表明，线虫虫卵是多参数流式细胞术分析的合适材料。前向散射发射是虫卵大小的鉴别参数。孵化率与侧向散射发射呈显著正相关。对于耐药菌株，耐药率与可能反映相关黄素分子氧化状态的天然绿色荧光脉冲呈显著回归关系。
