一种用于在细胞外记录中高分辨率检测突触后量子事件的算法。

An algorithm for high-resolution detection of postsynaptic quantal events in extracellular records.

作者信息

Bykhovskaia M, Worden M K, Hackett J T

机构信息

Department of Molecular Physiology, University of Virginia, Charlottesville 22908, USA.

出版信息

J Neurosci Methods. 1996 Apr;65(2):173-82. doi: 10.1016/0165-0270(95)00158-1.

DOI:10.1016/0165-0270(95)00158-1

PMID:8740595

Abstract

A software package has been developed for the detection and measurement of extracellularly recorded postsynaptic quantal events evoked by neural stimulation. The algorithm is based on the identification of monotonic regions of a differentiated current signal and detects the small inflections and peaks of a postsynaptic response that result from the asynchronous presynaptic release of individual packets of neurotransmitter. Recorded and simulated postsynaptic responses have been used to verify the accuracy of the algorithm and to determine its resolution. The algorithm can accurately detect up to six individual quanta in a time period of 20 ms, with a resolution of 0.5-1.0 ms.

摘要

已开发出一种软件包，用于检测和测量神经刺激诱发的细胞外记录的突触后量子事件。该算法基于对微分电流信号单调区域的识别，检测由单个神经递质包的异步突触前释放所导致的突触后反应的微小拐点和峰值。已使用记录的和模拟的突触后反应来验证该算法的准确性并确定其分辨率。该算法能够在20毫秒的时间段内准确检测多达六个单独的量子，分辨率为0.5 - 1.0毫秒。