Trerè D, Ceccarelli C, Danova M, Derenzini M
Dipartimento di Patologia Sperimentale, Università di Bologna, Italy.
Eur J Histochem. 1996;40(1):17-26.
The correlation between AgNOR (silver stained nucleolar organizer region) protein quantity and the percentage of S-phase cells was determined in 45 human tumours of different origin (28 from endometrium, 8 from cervix, 4 from ovary, 3 from larynx, 1 from rhinopharynx and 1 from tongue). AgNOR protein quantity was evaluated by image analysis on histological sections stained by the one-step silver staining method. S-phase cells were detected in histological sections with anti-bromo-deoxyuridine (BrdU) antibodies after in vivo BrdU infusion. The relationship between AgNOR protein and BrdU labelling index (LI) values was tested by linear regression analysis. A highly significant association of the two parameters was found (r = 0.78; p < 0.001), independent of the tumour type. DNA content of the 45 tumours was also determined by flow cytometry, and the DNA quantity was correlated with the kinetics data. Euploid tumours had a mean AgNOR protein area of 3.33 +/- 1.34 microns2 and a mean BrdU-LI of 10.78 +/- 8.94%, while in aneuploid tumours the mean AgNOR protein area was 4.78 +/- 1.78 microns2 and the mean BrdU-LI 17.37 +/- 8.79%. Both AgNOR protein quantity and BrdU-LI were significantly higher in aneuploid than in euploid tumours (Student-t Test: p < 0.05 and p < 0.02 for AgNOR and BrdU values, respectively). However, despite this significant difference, a relevant overlap of AgNOR protein and BrdU-LI scores was found within both the aneuploid and euploid groups.
在45例不同来源的人类肿瘤（28例来自子宫内膜、8例来自宫颈、4例来自卵巢、3例来自喉、1例来自鼻咽、1例来自舌）中，测定了银染核仁组织区（AgNOR）蛋白量与S期细胞百分比之间的相关性。通过对采用一步银染法染色的组织切片进行图像分析来评估AgNOR蛋白量。在体内注入溴脱氧尿苷（BrdU）后，用抗BrdU抗体在组织切片中检测S期细胞。通过线性回归分析检验AgNOR蛋白与BrdU标记指数（LI）值之间的关系。发现这两个参数之间存在高度显著的关联（r = 0.78；p < 0.001），与肿瘤类型无关。还通过流式细胞术测定了这45例肿瘤的DNA含量，并将DNA量与动力学数据相关联。整倍体肿瘤的平均AgNOR蛋白面积为3.33±1.34平方微米，平均BrdU-LI为10.78±8.94%，而在非整倍体肿瘤中，平均AgNOR蛋白面积为4.78±1.78平方微米，平均BrdU-LI为17.37±8.79%。非整倍体肿瘤中的AgNOR蛋白量和BrdU-LI均显著高于整倍体肿瘤（Student-t检验：AgNOR和BrdU值分别为p < 0.05和p < 0.02）。然而，尽管存在这一显著差异，但在非整倍体和整倍体组中均发现AgNOR蛋白和BrdU-LI评分有相当程度的重叠。
