Van Heeke G, Ott T L, Strauss A, Ammaturo D, Bazer F W
Department of Animal Science, Texas A&M University, College Station 77843-2471, USA.
J Interferon Cytokine Res. 1996 Feb;16(2):119-26. doi: 10.1089/jir.1996.16.119.
The early conceptus (embryo and associated membranes) of domestic ruminats signals its presence to the maternal uterus through production of interferon-tau (IFN-tau). Production of IFN-tau ensures continued production of progesterone, the hormone of pregnancy, by the ovarian corpus luteum. This paper reports the high-level expression and efficient secretion of biologically active recombinant ovine IFN-tau (rOvIFN-tau) by Pichia pastoris. The developed method produces more than 80% pure recombinant ovine IFN-tau, obviating the need for further purification for many purposes. Initial fermentation studies produced IFN-tau at 280 mg/liter and demonstrate the potential of this system for large-scale production of IFN-tau.
家养反刍动物的早期孕体（胚胎及相关膜）通过产生干扰素-τ（IFN-τ）向母体子宫发出其存在的信号。IFN-τ的产生可确保卵巢黄体持续分泌孕酮，即妊娠激素。本文报道了毕赤酵母高效表达并分泌具有生物活性的重组羊IFN-τ（rOvIFN-τ）。所开发的方法可产生纯度超过80%的重组羊IFN-τ，许多情况下无需进一步纯化。初步发酵研究表明，IFN-τ的产量可达280毫克/升，证明了该系统大规模生产IFN-τ的潜力。
