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[对在黑腹果蝇中诱导镶嵌型位置效应的T(1;2)dor(var7)易位断点区域的DNA序列分析]

[An analysis of the DNA sequence in the region of the breakpoint of the T(1;2)dor(var7) translocation inducing the mosaic-type position effect in Drosophila melanogaster].

作者信息

Makunin I V, Zakharkin S O, Kokoza E V, Pokholkova G V, Kholodilov N G, Zhimulev I F

出版信息

Tsitol Genet. 1996 Jan-Feb;30(1):8-14.

PMID:8743012
Abstract

The sites of location of (AC/GT) minisatellite sequence were detected in 2B region of X-chromosome of Drosophila melanogaster. The distribution of (AC/GT) repeats coincided with the location of one-half of the mapped breakpoints of known rearrangements within this region. Analysis of sequences neighbouring the breakpoints of the rearrangement T(1;2)dor(var7) demonstrated that the breaks between two sequences of (AC/GT) repeats occurred at a distance 80 and 156 bp, respectively, but there were no (AC/GT) repeats in r combination directly. T-rich sequences 25 bp long as well as short tandem repeats were found near the breakpoints. The pentanucleotide repeat (CTGTT)10 is located at a distance of 660 bp from the breakpoint. It differs by one nucleotide from the sequence of the GTGTT satellite attached to the 1A-2B fragment of X-chromosome as a result of rearrangement.

摘要

在黑腹果蝇X染色体的2B区域检测到了(AC/GT)小卫星序列的定位位点。(AC/GT)重复序列的分布与该区域内已知重排的一半映射断点的位置相吻合。对重排T(1;2)dor(var7)断点附近序列的分析表明,(AC/GT)重复序列的两个序列之间的断裂分别发生在80和156 bp的距离处,但r组合中没有直接的(AC/GT)重复序列。在断点附近发现了25 bp长的富含T的序列以及短串联重复序列。五核苷酸重复序列(CTGTT)10位于距断点660 bp处。由于重排,它与附着在X染色体1A - 2B片段上的GTGTT卫星序列的一个核苷酸不同。

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