Fierro R, Foliguet B, Grignon G, Daniel M, Bene M C, Faure G C, Barbarino-Monnier P
Depto. Ciencias Salud, Univ. Autónoma Metropolitana-Iztapalapa, México, Mexico.
Arch Androl. 1996 May-Jun;36(3):187-96. doi: 10.3109/01485019608987095.
Biochemical surface modifications occur during the capacitation and acrosome reaction of human sperm and among those, variations in the expression of carbohydrates moieties. A sequential study was performed with electronic microscopy and flow cytometry techniques, where the binding of 4 lectins was assessed on normal human sperm samples during in vitro induction of the acrosome reaction with calcium ionophore A-23187. Triticum vulgaris agglutinin (WGA) was shown to bind strongly the whole surface of sperm before induction of the acrosome reaction, and in lesser amounts after incubation with calcium ionophore. Arachis hypogea agglutinin (PNA) and mostly Concanavalia ensiformis agglutinin (Con-A) and Ulex europaeus agglutinin (UEA-1) binding evolved in an opposite pattern with an increase of the labeling parallel to that of GB24 antibody binding. Electron microscopy showed that the fluorescence patterns observed correlated with increased access to the inner membrane of the acrosome. This was significant 60 min after the induction of acrosome reaction. Lectin binding could be a useful tool to examine the ability of sperm samples to undergo the acrosome reaction.
生化表面修饰发生在人类精子的获能和顶体反应过程中，其中碳水化合物部分的表达存在变化。采用电子显微镜和流式细胞术进行了一项序贯研究，在使用钙离子载体A-23187体外诱导顶体反应期间，评估了4种凝集素在正常人精子样本上的结合情况。结果显示，普通小麦凝集素（WGA）在顶体反应诱导前能强烈结合精子的整个表面，而与钙离子载体孵育后结合量减少。花生凝集素（PNA）以及主要是刀豆球蛋白A（Con-A）和欧洲荆豆凝集素（UEA-1）的结合呈现相反的模式，其标记增加与GB24抗体结合的增加平行。电子显微镜显示，观察到的荧光模式与顶体内膜的可及性增加相关。这在顶体反应诱导60分钟后很明显。凝集素结合可能是检测精子样本进行顶体反应能力的有用工具。
