Zakeri Z, Quaglino D, Latham T, Woo K, Lockshin R A
Department of Biology, Queens College, Flushing, New York 11367, USA.
Microsc Res Tech. 1996 Jun 15;34(3):192-201. doi: 10.1002/(SICI)1097-0029(19960615)34:3<192::AID-JEMT2>3.0.CO;2-S.
The metamorphic death of the labial glands of the tobacco hornworm, Manduca sexta, occurs during a 4 day period during larva-to-pupa metamorphosis. The earliest changes marking the death of the cell, all occurring on the first day, are a sharp drop in protein synthesis, coupled with the selective survival or upregulation of a few messages. An early rearrangement of the rough endoplasmic reticulum is presumably related to the generalized decrease in protein synthesis. Lysosomal acid phosphatase also begins to increase very early, and ultimately the bulk of the cytoplasm is destroyed in autophagic vacuoles, but activation of lysosomes does not account for the decreased rate of synthesis. The mechanism by which most protein synthesis is depressed remains under investigation.
烟草天蛾（Manduca sexta）唇腺的变质性死亡发生在幼虫到蛹的变态过程中的4天内。标志着细胞死亡的最早变化都发生在第一天，包括蛋白质合成急剧下降，同时少数信使RNA选择性存活或上调。粗面内质网的早期重排可能与蛋白质合成的普遍下降有关。溶酶体酸性磷酸酶也很早就开始增加，最终大部分细胞质在自噬泡中被破坏，但溶酶体的激活并不能解释合成速率的下降。大多数蛋白质合成受到抑制的机制仍在研究中。
