Ichiki T, Kambayashi Y, Inagami T
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Kidney Int Suppl. 1996 Jun;55:S14-7.
Although the fetal aorta expresses a substantial amount of angiotensin II type 2 receptors, the expression level of angiotensin II type 2 receptors in the adult aorta and cultured vascular smooth muscle cells is very low or even absent. Prolonged serum depletion (6 to 8 days) with a supplement of insulin, transferrin and sodium selenite induced angiotensin II type 2 receptors and mRNA in cultured vascular smooth muscle cells from Wistar Kyoto rats. Insulin was found to be essential for the induction of the receptor. However, these receptors could not be induced in cultured vascular smooth muscle cells from spontaneously hypertensive rats. These results suggest that: (1) insulin plays an important role for the expression of the angiotensin II type 2 receptor gene; and (2) the type 2 receptor gene expression is differentially regulated between cultured vascular smooth muscle cells of Wistar Kyoto rats and spontaneously hypertensive rats.
虽然胎儿主动脉表达大量的血管紧张素II 2型受体，但在成年主动脉和培养的血管平滑肌细胞中，血管紧张素II 2型受体的表达水平非常低甚至不存在。用胰岛素、转铁蛋白和亚硒酸钠补充剂进行长时间血清剥夺（6至8天）可诱导Wistar Kyoto大鼠培养的血管平滑肌细胞中的血管紧张素II 2型受体和mRNA。发现胰岛素对于受体的诱导至关重要。然而，在自发性高血压大鼠培养的血管平滑肌细胞中不能诱导出这些受体。这些结果表明：（1）胰岛素在血管紧张素II 2型受体基因的表达中起重要作用；（2）Wistar Kyoto大鼠和自发性高血压大鼠培养的血管平滑肌细胞之间，2型受体基因表达受到不同的调节。
