Naloxone decreases intake of 10% sucrose in preweanling rats.

To investigate the role of opioids in the mediation of sucrose intake in the preweanling rat pup, we measured the effect of naloxone on intake of pups licking 10% sucrose from the floor of a beaker (independent ingestion test) and of pups ingesting 10% sucrose that was continuously infused through an anterior, sublingual oral catheter (oral catheter test). Pups were tested only once to eliminate the possible effect of test experience. Pups were tested in the second postnatal week (PN7, 9, 10, 11, and 14 days) with naloxone (1 mg/kg) or vehicle controls. Fourteen-day-old pups were also tested with 0.1 and 0.5 mg/kg. Naloxone began to be efficacious for inhibiting intake on PN10 in the oral catheter test and on PN11 in the independent ingestion test. On PN14, the inhibition of intake was dose related and naloxone was more potent for inhibiting intake in independent ingestion tests than in oral catheter tests. Naloxone not only decreased intake, it also decreased the incidence of licking, increased mouthing and resting, and had no significant effect on locomotion. The site of the inhibitory effect of naloxone on intake was in the central nervous system, presumably in the brain, because naloxonemethiodide, an analogue of naloxone that does not cross the blood-brain barrier, did not inhibit sucrose in either test. These results demonstrate that the intake of 10% sucrose depends on endogenous opioids as early as PN10 and that this opioid mechanism operates when pups have not had prior test experience and in a test (oral catheter test) where intake is not dependent on appetitive behaviors.