Drabick J A, Gracely E J, Heidecker G J, LiPuma J J
Department of Pathology, Medical College of Pennsylvania, Philadelphia, USA.
J Hosp Infect. 1996 Apr;32(4):267-76. doi: 10.1016/s0195-6701(96)90037-7.
Burkholderia (Pseudomonas) cepacia is an important pathogen amongst persons with cystic fibrosis (CF), and evidence suggests that transmission of strains within CF clinics contributes to pulmonary colonization of some patients. In order to optimize preventive strategies, the survival of B. cepacia on various environmental surfaces, including cotton cloth, stainless steel, latex and polyvinylchloride (PVC) tubing, was investigated. For surface inoculation, bacteria were suspended in phosphate buffered saline, sputum from CF patients, or sputum from persons without CF. The results demonstrate that amongst the strains examined, organisms survived significantly (P < 0.001) longer when suspended in sputum from CF patients than in either non-CF sputum or buffered saline. Significant (P < 0.001) differences in survival on the various surfaces were found; survival was greatest on PVC. Significant (P < 0.001) strain-to-strain differences in survival were also demonstrated; patient isolates representing predominant CF centre ribotypes survived longest. These data demonstrate that (1) B. cepacia can survive for long periods in respiratory droplets on environmental surfaces typically found in CF clinics, (2) undefined factors in sputum from patients with CF may contribute to survival of B. cepacia, and (3) strain-to-strain variation in survival time may affect strain transmissibility.
洋葱伯克霍尔德菌（假单胞菌属）是囊性纤维化（CF）患者中的一种重要病原体，有证据表明CF诊所内菌株的传播导致了一些患者的肺部定植。为了优化预防策略，研究了洋葱伯克霍尔德菌在各种环境表面（包括棉布、不锈钢、乳胶和聚氯乙烯（PVC）管材）上的存活情况。对于表面接种，将细菌悬浮于磷酸盐缓冲盐水、CF患者的痰液或非CF患者的痰液中。结果表明，在所检测的菌株中，当悬浮于CF患者的痰液中时，细菌的存活时间显著（P < 0.001）长于悬浮于非CF痰液或缓冲盐水中的存活时间。在不同表面上的存活情况存在显著（P < 0.001）差异；在PVC上的存活时间最长。还证明了菌株之间在存活时间上存在显著（P < 0.001）差异；代表主要CF中心核糖型的患者分离株存活时间最长。这些数据表明：（1）洋葱伯克霍尔德菌可在CF诊所常见的环境表面的呼吸道飞沫中长时间存活；（2）CF患者痰液中的未知因素可能有助于洋葱伯克霍尔德菌的存活；（3）存活时间的菌株间差异可能影响菌株的传播性。
