Kurowska E, Kochanowska I E, Kuropatwa M, Boratyński J, Szewczuk A
Biochemical Laboratory, Polish Academy of Sciences, Wrocław, Poland.
Arch Immunol Ther Exp (Warsz). 1995;43(3-4):181-5.
Characteristics of two monoclonal antibodies against human lutropin and their use in enzyme immunoassays of the hormone are presented. Diluted solution of pituitary lutropin was unstable in buffered saline and could be stabilized with some proteins. Concentration of the lutropin in woman urine, collected during "lutropin-peak", was markedly increased during long time storage at 5 degrees C or at room temperature. Using HPLC it was demonstrated that pituitary lutropin, normal urine lutropin and "increased" urinary lutropin were eluted from ion exchange column almost with the same retention time.
本文介绍了两种抗人促黄体生成素单克隆抗体的特性及其在该激素酶免疫测定中的应用。垂体促黄体生成素的稀释溶液在缓冲盐溶液中不稳定，可被某些蛋白质稳定。在“促黄体生成素峰值”期间收集的女性尿液中促黄体生成素的浓度，在5℃或室温下长时间储存时显著增加。使用高效液相色谱法表明，垂体促黄体生成素、正常尿液促黄体生成素和“升高的”尿液促黄体生成素从离子交换柱上洗脱下来的保留时间几乎相同。
