环孢素A的水解：通过快原子轰击串联质谱法鉴定1,11-断-环孢素A和4,5-断-异环孢素A

Hydrolysis of cyclosporin A: identification of 1,11 seco-cyclosporin A and 4,5 seco-isocyclosporin A by FAB-MS/MS.

作者信息

Magni F, Arnoldi L, Del Puppo M, Galli Kienle M

机构信息

Scientific Institute H. San Raffaele, Milan, Italy.

出版信息

Peptides. 1995;16(8):1335-41. doi: 10.1016/0196-9781(95)02025-x.

DOI:10.1016/0196-9781(95)02025-x

PMID:8745041

Abstract

We have previously reported that treatment of CsA with aqueous HCI gives rise to the formation of a number of water-soluble compounds. Two of these were identified from their FAB-MS/MS spectra as open-chain nona- and decapeptides. We describe here the identification of two other main compounds deriving from the same treatment. Identification was rendered possible from the comparison of their FAB-MS/MS spectra with those of methyl and acetyl derivatives. The two compounds are water-soluble, open-chain undecapeptides corresponding to 1.11 seco-CsA and of 4.5 seco-isoCsA, respectively.

摘要

我们之前报道过，用盐酸水溶液处理环孢素A会产生多种水溶性化合物。其中两种通过快原子轰击串联质谱（FAB-MS/MS）谱被鉴定为开链九肽和十肽。我们在此描述从相同处理中得到的另外两种主要化合物的鉴定情况。通过将它们的FAB-MS/MS谱与甲基和乙酰基衍生物的谱进行比较，鉴定成为可能。这两种化合物是水溶性的开链十一肽，分别对应1.11 去甲环孢素A和4.5 去甲异环孢素A。