心房心肌细胞中肽基甘氨酸α-酰胺化单加氧酶mRNA的患病率和周转率

Prevalence and turnover of peptidylglycine alpha-amidating monooxygenase mRNA in atrial cardiomyocytes.

作者信息

Maltese J Y, Oyarce A M, Eipper B A

机构信息

Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore MD 21205, USA.

出版信息

J Mol Cell Cardiol. 1996 Jan;28(1):155-63. doi: 10.1006/jmcc.1996.0015.

DOI:10.1006/jmcc.1996.0015

PMID:8745223

Abstract

Peptidylglycine alpha-amidating monooxygenase (PAM), the enzyme responsible for the alpha-amidation of neuroendocrine peptides, is more prevalent in the atrium of the heart than in pituitary or brain. RNase protection assays indicate that PAM transcripts account for approximately 0.5% of the mRNA in the neonatal atrium and 0.06% of the mRNA in the neonatal ventricle. In primary atrial cardiomyocyte cultures PAM mRNA turns over slowly, with a half-life of approximately 20 h. Levels of PAM mRNA in primary atrial cardiomyocytes are increased to 16.5% of control upon treatment with dexamethasone and decreased to 63% of control upon treatment with thyroid hormone.

摘要

肽基甘氨酸α-酰胺化单加氧酶（PAM）是负责神经内分泌肽α-酰胺化的酶，在心脏心房中的含量比在垂体或大脑中更高。核糖核酸酶保护分析表明，PAM转录本约占新生心房mRNA的0.5%，占新生心室mRNA的0.06%。在原代心房心肌细胞培养物中，PAM mRNA周转缓慢，半衰期约为20小时。用地塞米松处理后，原代心房心肌细胞中PAM mRNA的水平增加至对照的16.5%，用甲状腺激素处理后降至对照的63%。