Ito T, Kodama M
Hokkaido Branch, National Institute of Animal Health, Sapporo, Japan.
Res Vet Sci. 1996 Jan;60(1):94-6. doi: 10.1016/s0034-5288(96)90140-x.
The expression of the mRNAs of the cytokines interleukin (IL)-1 alpha, IL-1 beta, IL-2, IL-4, IL-6, interferon (IFN) gamma, tumour necrosis factor (TNF) alpha, and granulocyte-macrophage colony-stimulating factor (GM-CSF) was demonstrated by the reverse transcription-polymerase chain reaction (RT-PCR) in bovine alveolar macrophages (AMs) and peripheral blood mononuclear cells (PBMCs). By the use of the RT-PCR, a specific and highly sensitive technique has been developed to analyse simultaneously the array of cytokine messenger RNAs in bovine AMs and PBMCs. AMs stimulated with lipopolysaccaride (LPS) for four hours in vitro expressed IL-1 alpha, IL-1 beta, IL-6, TNF alpha and GM-CSF; PBMCs stimulated with LPS for four hours expressed IL-1 alpha, IL-1 beta, IL-6, TNF alpha and GM-CSF and when stimulated with concanavalin A (ConA) expressed IL-2, IL-4, IFN gamma and GM-CSF efficiently.
通过逆转录聚合酶链反应（RT-PCR）在牛肺泡巨噬细胞（AM）和外周血单核细胞（PBMC）中证实了细胞因子白细胞介素（IL）-1α、IL-1β、IL-2、IL-4、IL-6、干扰素（IFN）γ、肿瘤坏死因子（TNF）α和粒细胞-巨噬细胞集落刺激因子（GM-CSF）的mRNA表达。利用RT-PCR，已开发出一种特异性高且灵敏的技术，用于同时分析牛AM和PBMC中细胞因子信使RNA的阵列。体外经脂多糖（LPS）刺激4小时的AM表达IL-1α、IL-1β、IL-6、TNFα和GM-CSF；经LPS刺激4小时的PBMC表达IL-1α、IL-1β、IL-6、TNFα和GM-CSF，而经伴刀豆球蛋白A（ConA）刺激时则高效表达IL-2、IL-4、IFNγ和GM-CSF。
