[The neurite-outgrowth promoting effect from Clarke's nucleus of cat spinal cord with spared dorsal root preparation under electro-acupuncture stimulation on dorsal root ganglion of chicken embryo].

A modification of the double-coverslip hanging-drop culture technique originated from Maximow was introduced in the present report. The neurite - outgrowth promoting effect from Clarke's nucleus of cat spinal cord with partially lumbosacral dorsal rhizotomy as well as acupuncture stimulation of the points located within the peripheral innervating field of the spared dorsal root was examined with the modified culture technique. Findings demonstrated that an increased neurite-outgrowth promoting effect appeared in Clarke's nucleus of cat spinal cord with partial deafferentation, and that electro-acupuncture stimulation could enhance the neurite-outgrowth promoting effect further. It suggested that the increment of the neurite-outgrowth promoting effect might be related to the collateral sprouting of spared dorsal root and to the sprouting-promotion effect of acupuncture stimulation.