Pierres A, Benoliel A M, Bongrand P
Laboratoire d'immunologie, INSERM U. 387, Marseille, France.
C R Acad Sci III. 1995 Dec;318(12):1191-6.
We describe an experimental approach yielding new information on the behavior of ligand-receptor bonds. Spherical particles of 1.4 microns radius were coated with anti-rabbit immunoglobulin monoclonal antibodies and deposited on surfaces derivatized with rabbit immunoglobulins. Brownian motion was studied. When particles where bound through multiple bonds, the mean square displacement during a 1 s interval was 0.0038 micron2 as compared to 0.245 micron2 for unbound particles. Under the same conditions, the mean square displacement of particules coated with limiting dilutions of binding sites and bound by single molecular bonds was 0.0774 micron2. Results are compatible with the concept that the latter particles behaved as spheres transiently bound to the substratum by links of 2.7 nm length, allowing brownian oscillations with an angular amplitude of 0.062 radian.
我们描述了一种实验方法，该方法可产生有关配体-受体键行为的新信息。半径为1.4微米的球形颗粒用抗兔免疫球蛋白单克隆抗体包被，并沉积在用兔免疫球蛋白衍生化的表面上。对布朗运动进行了研究。当颗粒通过多个键结合时，1秒间隔内的均方位移为0.0038平方微米，而未结合颗粒的均方位移为0.245平方微米。在相同条件下，用结合位点的极限稀释液包被并通过单分子键结合的颗粒的均方位移为0.0774平方微米。结果与以下概念相符：后一种颗粒的行为就像是通过长度为2.7纳米的链瞬时结合到基质上的球体，允许进行角振幅为0.062弧度的布朗振荡。
