Courvalin P, Goussard S, Grillot-Courvalin C
Unité des agents antibactériens, CNRS EP J0058, Paris, France.
C R Acad Sci III. 1995 Dec;318(12):1207-12.
Transfer of genetic information between phylogenetically remote bacterial genera [1], from bacteria to yeast [2] and from bacteria to plants [3] by plasmid conjugation has been described. However, direct DNA transfer from prokaryotes to mammalian cells has not yet been demonstrated. Certain bacterial species have evolved the ability to enter mammalian cells by inducing their own internalization [4]. We show that invasive strains of Shigella flexneri and Escherichia coli, that undergo lysis upon entry into mammalian cells because of impaired cell wall synthesis, can act as stable DNA delivery systems to their host. This direct gene transfer is efficient, of broad host cell range and the replicative or integrative vectors so delivered are stably inherited and expressed by the cell progeny. DNA delivery by abortive invasion of eukaryotic cells by bacteria is of potential interest for stimulation of mucosal immunity and for in vivo or ex vivo gene therapy of human diseases.
通过质粒接合在系统发育上遥远的细菌属之间进行遗传信息转移[1]，从细菌到酵母[2]以及从细菌到植物[3]的情况已有报道。然而，从原核生物到哺乳动物细胞的直接DNA转移尚未得到证实。某些细菌物种已进化出通过诱导自身内化进入哺乳动物细胞的能力[4]。我们发现，弗氏志贺菌和大肠杆菌的侵袭性菌株，由于细胞壁合成受损，在进入哺乳动物细胞后会发生裂解，它们可以作为向宿主稳定递送DNA的系统。这种直接基因转移效率高、宿主细胞范围广，如此递送的复制型或整合型载体可被细胞后代稳定遗传和表达。细菌对真核细胞的流产性侵袭介导的DNA递送，对于刺激黏膜免疫以及人类疾病的体内或体外基因治疗具有潜在意义。
