Blandino T, Barrera M, Alonso M, Faure M, Rodriguez Diego J G
Laboratorio de Parasitología, Centro Nacional de Sanidad Agropecuaria, La Havane, Cuba.
Rev Elev Med Vet Pays Trop. 1995;48(3):244-6.
An immunoperoxidase assay for the serological diagnosis of Babesia bigemina was developed. The antigen slides were prepared from B. bigemina-infected blood and stored at -20 degrees C. One hundred and sixty five sera were tested, comparing the immunoperoxidase assay to the indirect fluorescent antibody test. A coincidence of 95% was observed between both tests. For the immunoperoxidase assay, a relative sensitivity of 94.8%, a relative specificity of 95.5%, and a positive predictive value of 96.8% were calculated. The results demonstrated the efficacy of this technique for detecting antibodies to B. bigemina.
