Kavanagh G M, Burton J L, Donnell V O
Department of Dermatology, Bristol Royal Infirmary, UK.
Br J Dermatol. 1996 Feb;134(2):234-7.
The effect of dithranol on superoxide generation in vivo has not been studied previously. Neutrophils produce large amounts of superoxide following stimulation by phorbol 12-myristate-13-acetate (PMA). We studied the effect of dithranol on PMA-activated superoxide generation by isolated neutrophils from normal subjects, and measured whole blood luminescence in response to PMA, using samples from 13 psoriatic patients before and during dithranol treatment. Oxidized dithranol had no effect on the PMA-stimulated superoxide generation in neutrophils, but there was a modest dose-related increase in superoxide generation by neutrophils exposed to dithranol before activation. Similar results were found with neutrophils obtained from the 13 psoriasis patients. Three of these patients suffered a dithranol burn and developed a neutrophilia, with a marked increase in stimulated superoxide generating ability. Our findings suggest that dithranol treatment of a psoriasis plaque is associated with a decrease in oxygen radical generating capacity in vivo. However, where inflammation of perilesional and uninvolved skin occurs, neutrophils appear to become semi-activated or primed.
此前尚未研究过地蒽酚对体内超氧化物生成的影响。中性粒细胞在佛波酯12 -肉豆蔻酸酯-13 -乙酸酯（PMA）刺激后会产生大量超氧化物。我们研究了地蒽酚对正常受试者分离出的中性粒细胞经PMA激活后超氧化物生成的影响，并使用13例银屑病患者在接受地蒽酚治疗前和治疗期间的样本，测量了全血对PMA反应的发光情况。氧化型地蒽酚对中性粒细胞中PMA刺激的超氧化物生成没有影响，但在激活前暴露于地蒽酚的中性粒细胞中，超氧化物生成有适度的剂量相关增加。从13例银屑病患者获得的中性粒细胞也得到了类似结果。其中3例患者发生了地蒽酚烧伤并出现了中性粒细胞增多，刺激后的超氧化物生成能力显著增加。我们的研究结果表明，地蒽酚治疗银屑病斑块与体内氧自由基生成能力降低有关。然而，在皮损周围和未受累皮肤发生炎症的部位，中性粒细胞似乎会变得半激活或致敏。
