慢性肝性脑病大鼠模型中突触体及脑片大脑皮质[3H]L-谷氨酸摄取情况

Synaptosomal and brain slice cerebrocortical [3H]L-glutamate uptake in a rat model of chronic hepatic encephalopathy.

作者信息

Maddison J E, Mickelthwaite C, Watson W E, Johnston G A

机构信息

Department of Pharmacology, University of Sydney, Australia.

出版信息

Neurochem Int. 1996 Jan;28(1):89-93. doi: 10.1016/0197-0186(95)00054-c.

DOI:10.1016/0197-0186(95)00054-c

PMID:8746768

Abstract

Cerebrocortical [3H]L-glutamate uptake was examined using brain slices and synaptosomes obtained from rats with portal vein and bile duct ligation. In addition, the effect of in vitro addition of 5 mM ammonia on glutamate uptake parameters was determined. There was no significant difference in brain slice or synaptosomal glutamate uptake in rats with portal vein and bile duct ligation compared to control rats. In vitro addition of ammonia had no effect on uptake kinetics in either brain slices or synaptosomes. These results suggest that glutamate uptake kinetics are not perturbed in this animal model of chronic hepatic encephalopathy.

摘要

使用从门静脉和胆管结扎大鼠获得的脑片和突触体检测脑皮质[3H]L-谷氨酸摄取。此外，测定了体外添加5 mM氨对谷氨酸摄取参数的影响。与对照大鼠相比，门静脉和胆管结扎大鼠的脑片或突触体谷氨酸摄取没有显著差异。体外添加氨对脑片或突触体的摄取动力学均无影响。这些结果表明，在这种慢性肝性脑病动物模型中，谷氨酸摄取动力学未受到干扰。