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Phosphorothioate oligodeoxynucleotides: antisense or anti-protein?

作者信息

Krieg A M, Stein C A

出版信息

Antisense Res Dev. 1995 Winter;5(4):241. doi: 10.1089/ard.1995.5.241.

DOI:10.1089/ard.1995.5.241
PMID:8746772
Abstract
摘要

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Phosphorothioate oligodeoxynucleotides: antisense or anti-protein?硫代磷酸酯寡脱氧核苷酸:反义还是抗蛋白质?
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Separation of synthetic phosphorothioate oligonucleotides from phosphodiester-defect species by strong-anion exchange HPLC.通过强阴离子交换高效液相色谱法从磷酸二酯缺陷型物种中分离合成的硫代磷酸酯寡核苷酸。
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Nucleotide pyrophosphatase/phosphodiesterase 1 is responsible for degradation of antisense phosphorothioate oligonucleotides.核苷酸焦磷酸酶/磷酸二酯酶1负责降解硫代磷酸酯反义寡核苷酸。
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Influence of diastereomeric ratios of deoxyribonucleoside phosphoramidites on the synthesis of phosphorothioate oligonucleotides.
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Modification of phosphorothioate oligonucleotides yields potent analogs with minimal toxicity for antisense experiments in the CNS.硫代磷酸酯寡核苷酸的修饰产生了对中枢神经系统反义实验毒性极小的有效类似物。
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Problems in interpretation of data derived from in vitro and in vivo use of antisense oligodeoxynucleotides.体外和体内使用反义寡脱氧核苷酸所获数据的解读问题。
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