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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Maxwell M H, Alexander I A, Robertson G W, Mitchell M A, McCorquodale C C

Roslin Institute (Edinburgh), Midlothian, Scotland.

Br Poult Sci. 1995 Dec;36(5):791-8. doi: 10.1080/00071669508417823.

The perfusion of livers with the vital dye trypan blue was performed to test for evidence of tissue hypoxia in 3 groups of young broiler chickens, namely, ascitic, hypoxia-induced and controls. 2. Hepatocytes that stained with trypan blue were considered to be dead or dying before fixation and represented damaged cells. 3. The proportion of trypan blue-stained hepatocytes in the livers of ascitic birds was slightly less than half that observed in the hypoxia-induced birds but significantly more than the proportion of stained cells observed in control birds. 4. Liver damage in the ascitic birds was also assessed biochemically by an altered enzyme profile. 5. The study demonstrated that increased trypan blue uptake in the livers of ascitic birds reared at sea-level may be the consequence of hypoxia stress caused by reduced oxygen utilisation.

对3组年轻肉鸡（即腹水型、低氧诱导型和对照组）的肝脏进行了活体染料台盼蓝灌注，以检测组织缺氧的证据。2. 用台盼蓝染色的肝细胞在固定前被认为已死亡或正在死亡，代表受损细胞。3. 腹水型鸡肝脏中被台盼蓝染色的肝细胞比例略低于低氧诱导型鸡，但明显高于对照组中被染色细胞的比例。4. 还通过改变的酶谱对腹水型鸡的肝脏损伤进行了生化评估。5. 该研究表明，在海平面饲养的腹水型鸡肝脏中台盼蓝摄取增加可能是由于氧利用减少导致的缺氧应激的结果。

Maxwell M H, Alexander I A, Robertson G W, Mitchell M A, McCorquodale C C

Roslin Institute (Edinburgh), Midlothian, Scotland.

Br Poult Sci. 1995 Dec;36(5):791-8. doi: 10.1080/00071669508417823.

The perfusion of livers with the vital dye trypan blue was performed to test for evidence of tissue hypoxia in 3 groups of young broiler chickens, namely, ascitic, hypoxia-induced and controls. 2. Hepatocytes that stained with trypan blue were considered to be dead or dying before fixation and represented damaged cells. 3. The proportion of trypan blue-stained hepatocytes in the livers of ascitic birds was slightly less than half that observed in the hypoxia-induced birds but significantly more than the proportion of stained cells observed in control birds. 4. Liver damage in the ascitic birds was also assessed biochemically by an altered enzyme profile. 5. The study demonstrated that increased trypan blue uptake in the livers of ascitic birds reared at sea-level may be the consequence of hypoxia stress caused by reduced oxygen utilisation.

对3组年轻肉鸡（即腹水型、低氧诱导型和对照组）的肝脏进行了活体染料台盼蓝灌注，以检测组织缺氧的证据。2. 用台盼蓝染色的肝细胞在固定前被认为已死亡或正在死亡，代表受损细胞。3. 腹水型鸡肝脏中被台盼蓝染色的肝细胞比例略低于低氧诱导型鸡，但明显高于对照组中被染色细胞的比例。4. 还通过改变的酶谱对腹水型鸡的肝脏损伤进行了生化评估。5. 该研究表明，在海平面饲养的腹水型鸡肝脏中台盼蓝摄取增加可能是由于氧利用减少导致的缺氧应激的结果。