Katsuragawa H, Kanzaki H, Inoue T, Hirano T, Narukawa S, Watanabe H, Mori T
Department of Gynaecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.
Hum Reprod. 1995 Nov;10(11):3028-34. doi: 10.1093/oxfordjournals.humrep.a135841.
We have developed an in-vitro co-culture system to examine the interaction between purified first trimester cytotrophoblasts and purified non-pregnant human endometrial stromal cells (ESC). ESC decidualization is an important step in endometrial maturation and may modulate embryo implantation. In order to investigate the effects of ESC decidualization on trophoblast function, we examined human chorionic gonadotrophin (HCG), human placental lactogen (HPL), progesterone and estrogen secretion by trophoblasts co-cultured in contact with ESC, either with or without decidualization induced by progesterone. Decidualized ESC inhibited basal HCG and HPL secretion for 3 days during the culture for HCG, and for 5 days during the culture for HPL (P < 0.01 and P < 0.03 respectively). After 5 days of co-culture, decidual transformation of ESC as indicated by prolactin production occurred in the control cultures due to progesterone and oestradiol secretion by the co-cultured trophoblasts, but no significant differences in HCG or HPL secretion were observed between the two groups. Although the type of trophoblast used in the present study is far from implantation, our results clearly demonstrated that HCG and HPL secretion by trophoblasts was inhibited by the presence of co-cultured decidualized ESC, and suggested that ESC decidualization may regulate trophoblast function at the human fetal-maternal interface.
我们开发了一种体外共培养系统，以研究纯化的孕早期细胞滋养层细胞与纯化的非孕人子宫内膜基质细胞（ESC）之间的相互作用。ESC蜕膜化是子宫内膜成熟的重要步骤，可能会调节胚胎着床。为了研究ESC蜕膜化对滋养层细胞功能的影响，我们检测了与ESC接触共培养的滋养层细胞分泌人绒毛膜促性腺激素（HCG）、人胎盘催乳素（HPL）、孕酮和雌激素的情况，ESC是否经孕酮诱导发生蜕膜化。在培养HCG的过程中，蜕膜化的ESC在3天内抑制了基础HCG分泌，在培养HPL的过程中，在5天内抑制了基础HPL分泌（分别为P < 0.01和P < 0.03）。共培养5天后，由于共培养的滋养层细胞分泌孕酮和雌二醇，对照组培养物中出现了以催乳素产生为指标的ESC蜕膜化转变，但两组之间在HCG或HPL分泌方面未观察到显著差异。尽管本研究中使用的滋养层细胞类型与着床相差甚远，但我们的结果清楚地表明，共培养的蜕膜化ESC的存在抑制了滋养层细胞分泌HCG和HPL，并提示ESC蜕膜化可能在人胎儿 - 母体界面调节滋养层细胞功能。
