Tarachand U, Pawse A R
Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Biochem Mol Biol Int. 1995 Dec;37(6):1037-45.
Artificially induced rat decidual tissue expresses plasminogen activator inhibitor (PAI). This PAI, isolated and purified employing chromatographic techniques, is a low molecular weight protein unlike the known PAIs. The final purified preparation resolves into a single band following SDS-PAGE and has an approximate molecular weight of 29 kDa. The properties studied include specificity for urokinase-type (uPA) and tissue-type (tPA) plasminogen activators, binding to conA and heparin, inhibition of thrombin, plasmin and trypsin. Decidual PAI is immunogenic in rabbit and a monospecific antiserum raised against the decidual inhibitor cross reacts with an extract of human placenta.
人工诱导的大鼠蜕膜组织表达纤溶酶原激活物抑制剂（PAI）。采用色谱技术分离纯化得到的这种PAI是一种低分子量蛋白质，与已知的PAI不同。最终纯化产物经SDS-PAGE后呈现为单一条带，其分子量约为29 kDa。所研究的特性包括对尿激酶型（uPA）和组织型（tPA）纤溶酶原激活物的特异性、与伴刀豆球蛋白A和肝素的结合、对凝血酶、纤溶酶和胰蛋白酶的抑制作用。蜕膜PAI在兔体内具有免疫原性，针对蜕膜抑制剂产生的单特异性抗血清与人类胎盘提取物发生交叉反应。
