Arinç E, Hanukoglu I, Sen A, Adali O
Department of Biology, Middle East Technical University, Ankara, Turkey.
Biochem Mol Biol Int. 1995 Dec;37(6):1121-6.
Expression of sheep lung microsomal cytochrome P4502B(LgM2) isozyme was determined in lung and liver preparations from rabbits, rats, sheep, cows and humans. Several tissues of sheep such as lung, liver, adrenal, kidney, ovary, intestine, muscle, spleen, pancreas and brain were also examined. Tissue homogenates were analyzed by Western-blotting with an antibody raised against purified sheep lung P4502B(LgM2) isozyme in rabbits. This isozyme was detected in lung samples from every species examined. Homologues of cytochrome P4502B(LgM2) were expressed in livers of sheep and rabbits but not in those of humans and cows. Although homologous forms of P4502B(LgM2) were not expressed in sheep brain, ovary, pancreas, spleen and muscle tissues, they were expressed in sheep adrenal, intestine and kidney.
在兔、大鼠、绵羊、牛和人的肺及肝组织样本中测定了绵羊肺微粒体细胞色素P4502B（LgM2）同工酶的表达情况。还检测了绵羊的多个组织，如肺、肝、肾上腺、肾、卵巢、肠、肌肉、脾、胰腺和脑。用针对在兔体内纯化的绵羊肺P4502B（LgM2）同工酶产生的抗体，通过蛋白质免疫印迹法对组织匀浆进行分析。在所检测的每个物种的肺样本中都检测到了这种同工酶。细胞色素P4502B（LgM2）的同源物在绵羊和兔的肝脏中表达，但在人和牛的肝脏中不表达。虽然P4502B（LgM2）的同源形式在绵羊脑、卵巢、胰腺、脾和肌肉组织中不表达，但在绵羊肾上腺、肠和肾中表达。
