Vriz S, Griffiths B L, Harley V, Goodfellow P, Lovell-Badge R
Unité de génétique des mammifères, Institut Pasteur, Paris, France.
Biochem Mol Biol Int. 1995 Dec;37(6):1137-46.
The sex-determining region of the Y chromosome gene, sry is expressed in the foetal mouse for a brief period, just before testis differentiation, which could be consistent with negative autoregulation. SRY is a DNA binding protein which can bind to cruciform DNA and to linear DNA with a sequence specificity. We have examined if the Sry gene contain DNA binding sites for the SRY protein itself. We have found that in an in vitro assay, the SRY protein binds to several sites of the Sry gene and especially to a (CA)25 sequence and to a (CAG)30 repeat. These binding suggest that the function of SRY and in a general way HMG-box proteins may be mediated by an interaction with repeat sequences.
Y染色体基因的性别决定区域，即Sry，在胎鼠中睾丸分化前的一段短暂时间内表达，这可能与负向自动调节相一致。SRY是一种DNA结合蛋白，它能以序列特异性结合十字形DNA和线性DNA。我们研究了Sry基因是否含有SRY蛋白自身的DNA结合位点。我们发现在体外试验中，SRY蛋白结合到Sry基因的几个位点，特别是一个(CA)25序列和一个(CAG)30重复序列。这些结合表明，SRY以及一般情况下HMG盒蛋白的功能可能是通过与重复序列的相互作用来介导的。
