Hoffman D J
J Pharm Sci. 1977 May;66(5):749-50. doi: 10.1002/jps.2600660542.
A rapid procedure for the determination of ibuprofen in human serum was developed using a single extraction with carbon tetrachloride after deproteinization with perchloric acid. The internal standard, 3-methyl-3-phenylbutyric acid, was added directly to the serum. Gas chromatograms were free of interfering peaks. Calibration curves (0--40 micron g/ml) were linear with a sensitivity of 0.5 micron of ibuprofen/ml of serum. Relative standard deviations ranged from 1.1 to 25%.
开发了一种快速测定人血清中布洛芬的方法，先用高氯酸使蛋白质沉淀，然后用四氯化碳单次萃取。内标物3-甲基-3-苯基丁酸直接加入血清中。气相色谱图无干扰峰。校准曲线（0 - 40微克/毫升）呈线性，血清中布洛芬的检测灵敏度为0.5微克/毫升。相对标准偏差范围为1.1%至25%。
