Isolation and characterization of a pseudogene related to human core 2 beta-1,6-N-acetylglucosaminyl-transferase.

In a previous study, we isolated genomic clones encoding core 2 beta-1,6-N-acetylglucosaminyltransferase (C2GnT) and blood group IGnT and proposed that these two genes were produced from a common ancestral gene by duplication, diversion and intron insertion. In the present study, we have isolated a pseudogene which is highly related to the gene of C2GnT. The sequence analysis of this pseudogene indicated that the pseudogene was produced by duplication of a common precursor gene for C2GnT. These results taken together strongly suggest that the ancestral gene was first duplicated and one of the duplicated genes directly evolved into the IGnT gene. The other duplicated gene was further duplicated to produce the C2GnT gene and the pseudogene.