Chen N, Terabe S
National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics.
Electrophoresis. 1995 Nov;16(11):2100-3. doi: 10.1002/elps.11501601340.
A quantitative study was made on the effect of organic modifiers in micellar electrokinetic chromatography (MEKC). A linear relationship between 1/k' and organic modifier concentration ([L]) at lower concentration range was derived and experimentally verified. This linearity was also examined at higher concentrations of organic modifier. The results showed that the linear relationship between 1/k' and [L] can only be observed under the situation that no significant change on micellar structure occurs upon the addition of organic modifiers. The mobility ratio of the solute which characterizes the properties of the solute was found to be mainly independent of [L]. A resolution equation for the neutral solutes was also derived in term of mobility ratio.
对胶束电动色谱法（MEKC）中有机改性剂的作用进行了定量研究。推导并通过实验验证了较低浓度范围内1/k'与有机改性剂浓度（[L]）之间的线性关系。还在较高浓度的有机改性剂下检验了这种线性关系。结果表明，只有在添加有机改性剂后胶束结构无显著变化的情况下，才能观察到1/k'与[L]之间的线性关系。发现表征溶质性质的溶质迁移率比主要与[L]无关。还根据迁移率比推导出了中性溶质的分离度方程。
