Altria K D, Clark B J, Filbey S D, Kelly M A, Rudd D R
Analytical Evaluation Group, Glaxo R & D, Ware, Herts, UK.
Electrophoresis. 1995 Nov;16(11):2143-8. doi: 10.1002/elps.11501601346.
Various chemometric experimental designs have been employed for the optimisation of capillary electrophoresis (CE) methods. Similar designs have been utilised in the assessment of the robustness of CE methods. The designs employed include central composites, fractional factorials, Plackett-Burman, simplex and overlapping-resolution mapping. Optimisation studies have largely concentrated on the use of these designs on selection of the optimal electrolyte composition. The robustness testing studies performed have involved the use of screening designs to identify the critical parameters affecting responses such as migration times and resolution. Further designs such as central composites have then been employed to set method limits following robustness studies. It is concluded that the use of experimental designs and statistical data evaluation in conjunction with personal computer-controlled CE autosamplers and instruments are of great benefit in the optimisation and robustness evaluation of CE methods.
各种化学计量学实验设计已被用于毛细管电泳（CE）方法的优化。类似的设计也被用于评估CE方法的稳健性。所采用的设计包括中心复合设计、分数析因设计、Plackett-Burman设计、单纯形设计和重叠分辨率映射。优化研究主要集中在将这些设计用于选择最佳电解质组成。所进行的稳健性测试研究涉及使用筛选设计来识别影响迁移时间和分辨率等响应的关键参数。然后，在稳健性研究之后，采用诸如中心复合设计等进一步的设计来设定方法限度。得出的结论是，将实验设计和统计数据评估与个人计算机控制的CE自动进样器和仪器结合使用，对CE方法的优化和稳健性评估非常有益。
