Blood pO2 along the rete of eel has been investigated with a microelectrode, the swim-bladder gas being replaced by air. 2. When the eel does not secrete gas, the pO2 profile is flat, whereas in actively secreting eel the pO2, along the rete has a maximum of approximately 1 atm near the bladder pole of the rete. 3. The findings confirm predictions of a mathematical model for counter current multiplication in the swim-bladder.