van Wilsem E J, Breve J, Savelkoul H, Claessen A, Scheper R J, Kraal G
Department of Cell Biology and Immunology, Vrije Universiteit, Amsterdam.
Immunobiology. 1995 Nov;194(4-5):403-14. doi: 10.1016/S0171-2985(11)80107-5.
In the skin and in the epithelium of the oral mucosa a comparable network of Langerhans cells can be found. Antigen application on either epithelium leads to rapid emigration of Langerhans cells to the draining lymph nodes. Application on the oral mucosa leads to tolerance induction while application on the skin results in sensitization of the animal. Here we show that there are no differences in the antigen presentation capacity of oral mucosa- and skin-derived dendritic cells. However, measurement of IFN-gamma and IL-5 production, as representatives of Th1 and Th2 cytokines respectively, in total lymph node suspensions after sensitization via the skin or oral mucosa demonstrated a skewing of the response towards Th2 after antigen application on the oral mucosa. Together with our previous studies, in which it was shown that oral tolerance induction is not inherent to oral mucosa-derived dendritic cells, we postulate that oral tolerance is determined at the level of draining lymph nodes influenced by local cytokine profiles.
在皮肤和口腔黏膜上皮中，可以发现类似的朗格汉斯细胞网络。在任何一种上皮上施加抗原都会导致朗格汉斯细胞迅速迁移至引流淋巴结。在口腔黏膜上施加抗原会诱导耐受，而在皮肤上施加抗原则会使动物致敏。我们在此表明，口腔黏膜来源和皮肤来源的树突状细胞在抗原呈递能力上没有差异。然而，通过皮肤或口腔黏膜致敏后，在总淋巴结悬液中分别测量作为Th1和Th2细胞因子代表的IFN-γ和IL-5的产生情况，结果显示在口腔黏膜上施加抗原后，反应倾向于Th2。结合我们之前的研究（其中表明口腔耐受诱导并非口腔黏膜来源的树突状细胞所固有），我们推测口腔耐受是由局部细胞因子谱影响的引流淋巴结水平所决定的。
