Serrano A, Garcia A, Abril E, Garrido F, Ruiz-Cabello F
Hospital Virgen de las Nieves, Universidad de Granada, Spain.
Immunobiology. 1995 Nov;194(4-5):449-56. doi: 10.1016/S0171-2985(11)80111-7.
The MAGE-1 gene encodes an antigen recognized on melanoma cells by autologous cytotoxic T cells. This gene shows a wide range of expression in many human tumors but not in normal tissues except for testes. We used reverse transcription polymerase chain reaction assays to analyze the expression of the MAGE-1 gene in two variants of an erythroleukemic cell line, K562. Comparison of two variants of the K562 cell line in different stages of differentiation showed different patterns of expression of the MAGE-1 gene. The more undifferentiated cell line (K562A) expressed high levels of specific MAGE-1 mRNA, in contrast to K562B, which features of erythroid differentiation, without MAGE-1 expression. Interestingly, we could not modulate MAGE-1 gene expression when in vitro differentiation of K562A was induced with Ara-C. Finally, our data indicate that MAGE-1 expression is not necessary for the maintenance of the transformed phenotype.
MAGE-1基因编码一种可被自身细胞毒性T细胞识别的黑色素瘤细胞抗原。该基因在许多人类肿瘤中广泛表达，但除睾丸外，在正常组织中不表达。我们使用逆转录聚合酶链反应分析了红白血病细胞系K562的两个变体中MAGE-1基因的表达。对处于不同分化阶段的K562细胞系的两个变体进行比较，发现MAGE-1基因的表达模式不同。与具有红系分化特征且不表达MAGE-1的K562B相比，分化程度较低的细胞系（K562A）表达高水平的特异性MAGE-1 mRNA。有趣的是，当用阿糖胞苷诱导K562A进行体外分化时，我们无法调节MAGE-1基因的表达。最后，我们的数据表明，MAGE-1的表达对于维持转化表型并非必需。
