通过在线固相萃取和柱液相色谱法快速、高度自动化测定血浆中的吗啡和吗啡葡糖醛酸化物。

Rapid and highly automated determination of morphine and morphine glucuronides in plasma by on-line solid-phase extraction and column liquid chromatography.

作者信息

Huwyler J, Rufer S, Küsters E, Drewe J

机构信息

Department of Anaesthesia and Research, University Hospital Basel, Switzerland.

出版信息

J Chromatogr B Biomed Appl. 1995 Dec 1;674(1):57-63. doi: 10.1016/0378-4347(95)00295-7.

DOI:10.1016/0378-4347(95)00295-7

PMID:8749252

Abstract

A high-performance liquid chromatography (HPLC) method has been developed for the determination of morphine and its main metabolites, morphine-6-glucuronide (M-6-G) and morphine-3-glucuronide (M-3-G), in plasma or cerebrospinal fluid. Samples were extracted using on-line solid-phase extraction followed by reversed-phase HPLC with fluorescence detection. Recoveries of 20 ng morphine and morphine glucuronides in plasma were over 95%. The limit of detection using 400 microliters of a biological matrix was 0.85, 3.4 and 1.0 ng/ml of M-3-G, M-6-G and morphine, respectively. Inter- and intra-day assay precision was better than 10%. The main advantages of the present described method are increased recoveries (> 95%) and a high degree of automation allowing a high speed in routine analysis. The time required for the fully automated analysis of one sample was less than 26 min.

摘要

已开发出一种高效液相色谱（HPLC）方法，用于测定血浆或脑脊液中的吗啡及其主要代谢物吗啡 - 6 - 葡萄糖醛酸苷（M - 6 - G）和吗啡 - 3 - 葡萄糖醛酸苷（M - 3 - G）。样品采用在线固相萃取，然后进行反相HPLC并采用荧光检测进行提取。血浆中20 ng吗啡和吗啡葡萄糖醛酸苷的回收率超过95%。使用400微升生物基质时，M - 3 - G、M - 6 - G和吗啡的检测限分别为0.85、3.4和1.0 ng/ml。日内和日间测定精密度优于10%。所述方法的主要优点是回收率提高（>95%）以及自动化程度高，可实现常规分析的高速化。一个样品的全自动分析所需时间少于26分钟。