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用于细胞外记录突触电位的碳纤维微电极的制备

Preparation of carbon-fibre microelectrode for extracellular recording of synaptic potentials.

作者信息

Kuras A, Gutmaniene N

机构信息

Laboratory of Neurophysiology, Kaunas Medical Academy, Lithuania.

出版信息

J Neurosci Methods. 1995 Nov;62(1-2):207-12. doi: 10.1016/0165-0270(95)00078-x.

DOI:10.1016/0165-0270(95)00078-x
PMID:8750105
Abstract

A method is proposed for rapid and effective forming of the recording tips of carbon-fibre microelectrodes by trimming and treating the tip with electric current pulses. The tip is trimmed to the required length. The signal-to-noise ratio of carbon-fibre microelectrodes is improved at low-frequency range (less than 100 Hz) after the treatment. The same microelectrode may be used for several experiments because of the possibility to trim and treat the tips repeatedly. This method also allows one to readily fabricate 2- or multi-channel carbon-fibre microelectrodes with the vertical distance between recording tips from few tens to hundreds of micrometres. As an example, the process of fabrication of a 2-channel microelectrode is described.

摘要

提出了一种通过修剪和用电流脉冲处理尖端来快速有效地形成碳纤维微电极记录尖端的方法。将尖端修剪到所需长度。处理后,碳纤维微电极在低频范围(小于100Hz)的信噪比得到改善。由于可以重复修剪和处理尖端,同一微电极可用于多个实验。该方法还允许人们轻松制造记录尖端之间垂直距离从几十到几百微米的双通道或多通道碳纤维微电极。作为示例,描述了双通道微电极的制造过程。

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