急性淋巴细胞白血病患者外周血干细胞采集中克隆性免疫球蛋白基因重排的检测
Detection of clonal immunoglobulin gene rearrangements in the PBPC harvests of patients with acute lymphoblastic leukaemia.
作者信息
Owen R G, Johnson R J, Evans P A, Smith G M, Child J A, Morgan G J
机构信息
Centre for Haematological Oncology, General Infirmary, Leeds, UK.
出版信息
Bone Marrow Transplant. 1995 Dec;16(6):831-4.
We have used a fluorescently based PCR technique to detect rearrangements in the immunoglobulin heavy chain (IgH) gene in the presentation BM of five patients with adult ALL and have looked for similar rearrangements in their PBPC. Using this approach we have been able to demonstrate clonal rearrangements in the PBPC of two of five patients. Remission BM samples taken 6-12 weeks prior to leucapheresis failed to show a clonal rearrangement in either patient. The significance of these results is discussed.
我们使用了一种基于荧光的聚合酶链反应（PCR）技术来检测5例成人急性淋巴细胞白血病（ALL）患者初诊骨髓（BM）中免疫球蛋白重链（IgH）基因的重排，并在其外周血祖细胞（PBPC）中寻找类似的重排。通过这种方法，我们能够证明5例患者中有2例的PBPC存在克隆性重排。在白细胞单采前6 - 12周采集的缓解期BM样本中，这两名患者均未显示克隆性重排。本文讨论了这些结果的意义。
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