Aust G, Lehmann I, Heberling H J
Department of Internal Medicine III, University of Leipzig, Germany.
Exp Clin Endocrinol Diabetes. 1996;104(1):50-8. doi: 10.1055/s-0029-1211422.
Direct multi-colour flow cytometric analysis was employed in patients with Graves' disease (n = 10) to determine the immunophenotype in peripheral blood lymphocytes (PBL) at the time of diagnosis without treatment (PBLw) and prior to operation (PBLp) and in thyroid-derived lymphocytes (TL). Additionally, the secretion of anti-thyroperoxidase antibodies (anti-TPO) was measured during culture of isolated peripheral or thyroid-derived B cells. Among TL from patients with high serum levels of anti-TPO (6/10) a significantly (p < 0.01) higher percentage of B cells were detected compared to PBLp (TL: 21.7 +/- 7.2%; PBLp: 13.2 +/- 4.5%). Enriched thyroid-derived B cells only from these patients also showed high spontaneous anti-TPO secretion during culture. The difference between peripheral and thyroid-derived natural killer (NK) cells was highly significant (p < 0.001; TL: 5.6 +/- 6.3%; PBLp: 13.6 +/- 5.5%). Two patients were found with a higher number of NK cells within TL. These patients were among those who had a low number of B cells infiltrating the thyroid gland. Regarding the expression of several other differentiation antigens, i.e. CD4 and CD8, gamma/delta TCR bearing T cells and CD45R0 on CD4+ T cells as a marker for memory cells, on TL no differences could be detected between patients with or without anti-TPO. In TL 31.5 +/- 7.7% of CD3- cells expressed the HLA-DR antigen (vs. 6.1 +/- 2.4% in PBLp; p < 0.001). Half of these cells simultaneously expressed the activation antigen CD69. Surprisingly, the number of CD3+ TL bearing the IL-2 receptor (CD25) and transferrin receptor (CD71) was not increased. Taken together, the proportional distribution of B and NK cells within the thyroid correlates with the anti-TPO secretion in vivo and in vitro, suggesting different immune response regulation processes of TL.
对10例格雷夫斯病患者进行直接多色流式细胞术分析，以确定未经治疗的诊断时（PBLw）、手术前（PBLp）外周血淋巴细胞（PBL）以及甲状腺来源淋巴细胞（TL）的免疫表型。此外，在分离的外周或甲状腺来源B细胞培养期间测量抗甲状腺过氧化物酶抗体（抗TPO）的分泌。在抗TPO血清水平高的患者（6/10）的TL中，与PBLp相比，检测到的B细胞百分比显著更高（p < 0.01）（TL：21.7 +/- 7.2%；PBLp：13.2 +/- 4.5%）。仅来自这些患者的富集甲状腺来源B细胞在培养期间也显示出高自发抗TPO分泌。外周和甲状腺来源的自然杀伤（NK）细胞之间的差异非常显著（p < 0.001；TL：5.6 +/- 6.3%；PBLp：13.6 +/- 5.5%）。发现2例患者的TL中NK细胞数量较多。这些患者属于甲状腺中浸润B细胞数量较少的患者。关于其他几种分化抗原的表达，即CD4和CD8、携带γ/δTCR的T细胞以及作为记忆细胞标志物的CD4 + T细胞上的CD45R0，在有或没有抗TPO的患者的TL之间未检测到差异。在TL中，31.5 +/- 7.7%的CD3 - 细胞表达HLA - DR抗原（相比之下，PBLp中为6.1 +/- 2.4%；p < 0.001）。这些细胞中有一半同时表达激活抗原CD69。令人惊讶的是，携带白细胞介素 - 2受体（CD25）和转铁蛋白受体（CD71）的CD3 + TL数量没有增加。总之，甲状腺内B细胞和NK细胞的比例分布与体内和体外抗TPO分泌相关，提示TL存在不同的免疫反应调节过程。
