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琼脂凝胶沉淀试验、对流免疫电泳和被动血凝试验在绵羊和山羊肝片吸虫感染诊断中的比较评价

Comparative evaluation of agar gel precipitation, counterimmunoelectrophoresis and passive haemagglutination tests for the diagnosis of Dicrocoelium dendriticum infection in sheep and goats.

作者信息

Jithendran K P, Vaid J, Krishna L

机构信息

Disease Investigation Laboratory, Indian Veterinary Research Institute, Palampur, India.

出版信息

Vet Parasitol. 1996 Jan;61(1-2):151-6. doi: 10.1016/0304-4017(95)00808-x.

DOI:10.1016/0304-4017(95)00808-x
PMID:8750692
Abstract

The sensitivity and specificity of the agar gel precipitation test (AGPT), counter immunoelectrophoresis (CIEP) and passive haemagglutination test (PHT) were evaluated for the diagnosis of Dicrocoelium dendriticum infection in naturally infected sheep and goats. Two hundred and forty five sheep and goat sera samples were tested using phosphate buffered saline, pH 7.2 extracted adult fluke antigen. CIEP detected 69.8% of the infected animals and was found to be the most sensitive, followed by PHT which detected 50.0% of the infected sheep and goats. AGPT was found to be the least sensitive, detecting only 23.8% of the infected animals. The specificity of

摘要

对琼脂凝胶沉淀试验(AGPT)、对流免疫电泳(CIEP)和被动血凝试验(PHT)在诊断自然感染的绵羊和山羊肝片吸虫感染中的敏感性和特异性进行了评估。使用pH 7.2的磷酸盐缓冲盐水提取的成虫吸虫抗原对245份绵羊和山羊血清样本进行了检测。CIEP检测出69.8%的感染动物,被发现是最敏感的,其次是PHT,检测出50.0%的感染绵羊和山羊。AGPT被发现是最不敏感的,仅检测出23.8%的感染动物。……的特异性

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