Volsen S G, Day N C, McCormack A L, Smith W, Craig P J, Beattie R, Ince P G, Shaw P J, Ellis S B, Gillespie A
Lilly Research Centre Limited, Eli Lilly and Company, Surrey, UK.
Brain Res Mol Brain Res. 1995 Dec 28;34(2):271-82. doi: 10.1016/0169-328x(95)00234-j.
Little is known about the comparative distribution of voltage-dependent calcium channel subtypes in normal human brain. Previous studies in experimental animals have predominantly focused on the regional expression of single alpha 1 genes. We describe the preparation of riboprobes and antisera specific for human alpha 1A, alpha 1B and alpha 1E subunits and their application in comprehensive mapping studies of the human cerebellum. Within the cerebellar cortex, these pore forming proteins were found to have differential localisations when examined in adjacent sections. The alpha 1A and alpha 1B subunits broadly colocalised and were both present, though at apparently different levels, in the molecular, Purkinje and granule cell layers whilst alpha 1E was predominantly expressed in Purkinje cells. In the dentate nucleus, an area which has received little attention in previous studies, alpha 1A was highly expressed in regions in which Purkinje cell nerve terminals form synapses with deep cerebellar neurones.
关于电压依赖性钙通道亚型在正常人类大脑中的比较分布，我们知之甚少。先前在实验动物身上进行的研究主要集中在单个α1基因的区域表达上。我们描述了针对人类α1A、α1B和α1E亚基的核糖探针和抗血清的制备及其在人类小脑综合图谱研究中的应用。在小脑皮质内，当在相邻切片中检查时，发现这些形成孔道的蛋白质具有不同的定位。α1A和α1B亚基广泛共定位，并且在分子层、浦肯野细胞层和颗粒细胞层中均有表达，尽管表达水平明显不同，而α1E主要在浦肯野细胞中表达。在齿状核中，这是一个在先前研究中很少受到关注的区域，α1A在浦肯野细胞神经末梢与小脑深部神经元形成突触的区域高度表达。
