O'Connor L T, Lauterborn J C, Smith M A, Gall C M
Department of Anatomy and Neurobiology, University of California at Irvine 92717-1275, USA.
Brain Res Mol Brain Res. 1995 Nov;33(2):277-87. doi: 10.1016/0169-328x(95)00147-k.
Agrin mRNA is broadly distributed throughout the adult rat brain, consistent with its proposed role in synaptogenesis and the organization of synaptic proteins in the central nervous system. The present study examined the effect of neuronal activity on agrin mRNA expression in adult rat forebrain using the hilus lesion paradigm for seizure induction and in situ hybridization and polymerase chain reaction techniques for quantification and characterization of agrin mRNA content. Seizures induced rapid, prolonged, and region-specific changes in agrin mRNA expression with the most prominent alterations occurring in hippocampal and cortical neurons. However, there were no detectable perturbations in the relative abundance of alternatively spliced agrin transcripts in affected brain regions. Activity-dependent changes in agrin expression suggest a role for this protein in modifications of synaptic structure associated with functional synaptic plasticity.
聚集蛋白聚糖信使核糖核酸广泛分布于成年大鼠大脑中，这与其在突触形成以及中枢神经系统中突触蛋白组织方面所假定的作用相一致。本研究采用海马损伤模型诱导癫痫发作，并运用原位杂交和聚合酶链反应技术来定量和鉴定聚集蛋白聚糖信使核糖核酸的含量，以此探究神经元活动对成年大鼠前脑聚集蛋白聚糖信使核糖核酸表达的影响。癫痫发作引发了聚集蛋白聚糖信使核糖核酸表达快速、持久且具有区域特异性的变化，其中最显著的改变发生在海马体和皮质神经元中。然而，在受影响的脑区中，未检测到选择性剪接的聚集蛋白聚糖转录本的相对丰度有任何扰动。聚集蛋白聚糖表达的活动依赖性变化表明，该蛋白在与功能性突触可塑性相关的突触结构修饰中发挥作用。
