Boué-Grabot E, Dufy B, Garret M
Laboratoire de Neurophysiologie, CNRS URA 1200, Université de Bordeaux II, France.
Brain Res. 1995 Dec 15;704(1):125-9. doi: 10.1016/0006-8993(95)01192-7.
mRNA expression of GABA-gated Cl(-)-channels in rat antepituitary was evaluated by using an reverse-transcribed (RT)-polymerase chain reaction (RT-PCR) method with degenerate and specific oligonucleotides. The main result of our findings is that the antepituitary expresses mRNAs encoding alpha 4 and rho 1 GABA receptor subunits. These two subunits are believed to be, respectively, constituents of benzodiazepine-insensitive GABAA and GABAC receptors in the CNS. This molecular analysis is consistent with the pharmacological diversity of GABA receptors in pituitary cells.
采用逆转录（RT）-聚合酶链反应（RT-PCR）方法，使用简并和特异性寡核苷酸，评估大鼠垂体前叶中γ-氨基丁酸（GABA）门控氯离子通道的mRNA表达。我们研究结果的主要发现是，垂体前叶表达编码α4和ρ1 GABA受体亚基的mRNA。这两个亚基分别被认为是中枢神经系统中对苯二氮䓬不敏感的GABAA和GABAC受体的组成部分。这种分子分析与垂体细胞中GABA受体的药理学多样性一致。
