Ekkel E D, Kuypers A H, Counotte G H, Tielen M J
Department of Herd Health and Reproduction, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
Vet Q. 1995 Dec;17(4):143-6. doi: 10.1080/01652176.1995.9694554.
The present study describes two experiments to standardize the phytohaemagglutinin (PHA) skin test as an indicator of lymphocyte reactivity in pigs after exposure to stressful situations in practical pig husbandry. The first experiment was a dose-response experiment. No time dose interaction was found, so the pattern of the reaction was the same for all doses used, although the magnitude of the reaction differed. The second experiment was carried out to determine stress-induced changes in lymphocyte reactivity. One half of a group of 48 pigs was exposed to stress (mixing, moving, and an increased stocking density). The immune reactivity to intradermally injected PHA of stressed animals differed significantly (P < 0.01) from that of unstressed controls. The reaction of stressed animals can be characterized by a delayed reaction with a lower peak. It can be concluded that frequent measurements are preferable to single measurements when the effects of stress on immunological processes are studied.
本研究描述了两项实验，旨在将植物血凝素（PHA）皮肤试验标准化，作为实际养猪生产中猪只暴露于应激状况后淋巴细胞反应性的指标。第一个实验是剂量反应实验。未发现时间-剂量相互作用，因此尽管反应强度不同，但所有使用剂量的反应模式相同。第二个实验旨在确定应激引起的淋巴细胞反应性变化。一组48头猪中的一半暴露于应激（混群、转移和饲养密度增加）。应激动物对皮内注射PHA的免疫反应性与未应激对照相比有显著差异（P < 0.01）。应激动物的反应特点是反应延迟且峰值较低。可以得出结论，在研究应激对免疫过程的影响时，频繁测量优于单次测量。
