Gillis-Haegerstrand C, Frebelius S, Haegerstrand A, Swedenborg J
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
J Vasc Surg. 1996 Aug;24(2):226-34.
Reduction of the thrombogenicity of synthetic vascular grafts by endothelialization has been suggested. The purpose of this study was to examine some of the non-thrombogenic properties of cultured adult human great saphenous vein endothelial cells seeded on expanded polytetrafluoroethylene (ePTFE) grafts.
Endothelialized grafts, control grafts, and wells were incubated with thrombin. Assays of thrombin loss from solution, thrombin coagulant activity, and protein C activation on the surface were obtained. The presence of thrombomodulin was confirmed with immunohistochemistry.
Significantly more thrombin was found on the ePTFE grafts or wells that underwent endothelialization, and larger amounts were lost from the thrombin solution compared with the control group. Thrombin enzyme activity on the endothelialization group was almost completely represented by activation of protein C and only to a minor extent by activity towards fibrinogen.
It is concluded that endothelial cells seeded on ePTFE retain the possibility to inhibit thrombin coagulant activity and to activate protein C. These findings provide support for the clinical applicability of cultured autologous endothelium on ePTFE grafts.
有人提出通过内皮化来降低合成血管移植物的血栓形成性。本研究的目的是检测接种于膨化聚四氟乙烯（ePTFE）移植物上的培养成人隐静脉内皮细胞的一些抗血栓形成特性。
将内皮化移植物、对照移植物和孔板与凝血酶一起孵育。检测溶液中凝血酶的损失、凝血酶的凝血活性以及表面蛋白C的激活情况。通过免疫组织化学确认血栓调节蛋白的存在。
与对照组相比，在内皮化的ePTFE移植物或孔板上发现的凝血酶明显更多，并且从凝血酶溶液中损失的量更大。内皮化组的凝血酶酶活性几乎完全由蛋白C的激活来体现，而对纤维蛋白原的活性仅占很小一部分。
得出的结论是，接种于ePTFE上的内皮细胞保留了抑制凝血酶凝血活性和激活蛋白C的可能性。这些发现为培养的自体内皮细胞在ePTFE移植物上的临床应用提供了支持。
